molecular epidemiology of systemic salmonella enterica serovar typhimurium outbreak in canaries
نویسندگان
چکیده
background and objectives: in may 2007, high mortality with severe septicemia was reported in 17 flocks of canaries in different regions of tehran province. this study was designed to follow up and study a great outbreak of salmonellosis in these canaries. materials and methods: two carcasses from every flock, environment, food and water resources were examined. after isolating the bacteria, serotyping and multiplex pcr were performed to confirm the bacteria identified. the isolates within the same serovars were investigated by r-typing using 33 antibiotics and then subjected to rapd-pcr with three primers. the genomic dna from these isolates were digested with xba і and the macro restriction fragment were separated by pfge. results: s . typhimurium was isolated from dead carcasses, visceral organs, stools and feed. thirty-six isolated strains (35 isolates from canary carcasses and one isolate from feed) showed similar results in all of the tests, confirming the occurrence of an outbreak. conclusion: canaries seem to be very susceptible to infection with s . typhimurium . the clonality of isolated organisms and its characteristics is significantly important due to the severe septicemia and high mortalities in this outbreak and its public health threats. environmental contamination within the cages, and food contaminated with stools of other canaries were the sources of infection. inspection for food hygiene, daily cleaning of canary’s cage from stool and carrier insects and rodents are necessary to prevent such outbreaks. combination of r-typing, rapd-pcr and pfge increase the differentiation power of isolates, however, they showed clonality of s. typhimurium involved in this outbreak.
منابع مشابه
Epidemiology of a Salmonella enterica subsp. enterica serovar Typhimurium strain associated with a songbird outbreak.
Salmonella enterica subsp. enterica serovar Typhimurium is responsible for the majority of salmonellosis cases worldwide. This Salmonella serovar is also responsible for die-offs in songbird populations. In 2009, there was an S. Typhimurium epizootic reported in pine siskins in the eastern United States. At the time, there was also a human outbreak with this serovar that was associated with con...
متن کاملGlobal Genomic Epidemiology of Salmonella enterica Serovar Typhimurium DT104
It has been 30 years since the initial emergence and subsequent rapid global spread of multidrug-resistant Salmonella entericaserovar Typhimurium DT104 (MDR DT104). Nonetheless, its origin and transmission route have never been revealed. We used whole-genome sequencing (WGS) and temporally structured sequence analysis within a Bayesian framework to reconstruct temporal and spatial phylogenetic ...
متن کاملMultilocus Sequence Typing of the Clinical Isolates of Salmonella Enterica Serovar Typhimurium in Tehran Hospitals
Background: Salmonella enterica serovar Typhimurium is one of the most important serovars of Salmonella enterica and is associated with human salmonellosis worldwide. Many epidemiological studies have focused on the characteristics of Salmonella Typhimurium in many countries as well as in Asia. This study was conducted to investigate the genetic characteristics of Salmonella Typhimurium using m...
متن کاملTriclosan resistance in Salmonella enterica serovar Typhimurium.
OBJECTIVES The aim of this study was to characterize the mechanisms of resistance to triclosan in Salmonella enterica serovar Typhimurium. METHODS Mutants resistant to triclosan were selected from nine S. enterica serovar Typhimurium strains. Mutants were characterized by genotyping, mutagenesis and complementation of fabI and analysis of efflux activity. Fitness of triclosan-resistant mutant...
متن کاملDetection of Salmonella enterica Serovar Typhimurium from Avians Using Multiplex-PCR
Salmonella enterica serovar Typhimurium and S.enterica serovar Enteritidis are the most frequently isolated serovars from food-borne diseases throughout the world. According to their antigenic profiles, salmonella shows different disease syndromes and host specificities. It is necessary and important to discriminate salmonella serovars from each other in order to ensure that each pathogen and i...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of microbiologyجلد ۱، شماره ۳، صفحات ۷-۱۱
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023